000			03100cam a2200337Ia 4500
001			CRC0KE13302PDF
003			BD-DhSAU
005			20151012144301.0
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007			cr||||
008			130725s2014 fluadf sbf 001 0 eng d
020			_a9781439869109 (ebook : PDF)
040			_aBD-DhSAU _cBD-DhSAU
090			_aQR186.7 _b.M35 2014
092			_a616.0798 _bM235
245	0	0	_aMaking and using antibodies _h[electronic resource] : _ba practical handbook / _cedited by Gary C. Howard and Matthew R. Kaser.
250			_a2nd ed.
260			_aBoca Raton : _bCRC Press, _c2014.
300			_axiv, 444 p., [2] p. of col. plates : _bill.
504			_aIncludes bibliographical references and index.
505	0		_ach. 1. Antibodies / Matthew R. Kaser and Gary C. Howard -- ch. 2. Antigens and adjuvants / Paul Algate, Jory Baldridge, and Sally P. Mossman -- ch. 3. Production of polyclonal antibodies / Lon V. Kendall -- ch. 4. Purification and characterization of antibodies / Joseph P. Chandler -- ch. 6. Production of monoclonal antibodies / Kathleen C.F. Sheehan -- ch. 6. Quantitative production of monoclonal antibodies / David A. Fox and Elizabeth M. Smith -- ch. 7. Making antibodies in bacteria / Frederic A. Fellouse and Sachdev S. Sidhu -- ch. 8. In vitro selection of aptamers / Tianjiao Wang, Xiangyu Cong, and Marit Nilsen-Hamilton -- ch. 9. Chemical and proteolytic modification of antibodies / George P. Smith -- ch. 10. Western blots and other applications / Lee Bendickson and Marit Nilsen-Hamilton -- ch. 11. Immunohistochemical methods / Jos�e A. Ramos-Vara -- ch. 12. Immunoelectron microscopy / Sara E. Miller and David N. Howell -- ch. 13. Flow cytometry / Steven B. McClellan -- ch. 14. ELISAs / John Chen and Gary C. Howard -- ch. 15. Humanization of antibodies / Juan Carlos Almagro, Sreekumar Kodangattil, and Jian Li -- ch. 16. Antibodies in the future : challenges and opportunities / Gary C. Howard and Matthew R. Kaser.
520			_a"This second edition provides guidance on all aspects of how to make and use antibodies for research, and commercial and industrial applications. All chapters are revised, updated, or expanded. New material in the book includes western blotting, aptamers, antibodies as therapeutics, quantitative production, and humanization of antibodies. The authors present descriptions of basic methods for making and using antibodies and describe basic laboratory techniques in detail. Each chapter includes introductory material, allowing for a better understanding of each concept. Practical examples are also included"--Provided by publisher.
530			_aAlso available in print edition.
538			_aMode of access: World Wide Web.
650		0	_aImmunoglobulins _vHandbooks, manuals, etc.
655		7	_aElectronic books. _2lcsh
700	1		_aHoward, Gary C.
700	1		_aKaser, Matthew R.
776	1		_z9781439869086 (pbk. : alk. paper)
856	4	0	_uhttp://marc.crcnetbase.com/isbn/9781439869109 _qapplication/PDF
999			_c11351 _d11350